Decrease of miR-622 expression suppresses migration and invasion by targeting regulation of DYRK2 in colorectal cancer cells

• DOI: 10.2147/ott.s125724
• Publication Date: 2017-02-22T20:38:28Z
• Authors: Yong Wang; Jie Sun; Xilin Wei; Lan Luan; Xiandong Zeng; Cuifang Wang; Wei Zhao
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

Background: More and more evidence indicates that microRNAs are present involved in many tumor-related diseases. The function of microRNA-622 (miR-622) colorectal cancer (CRC) remains controversial. Dual specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) has been reported as a tumor suppressor gene different cancers. detailed regulation mechanism DYRK2 CRC unclear. Methods: miR-622 expression levels were detected at tissue cellular level respectively by using real time polymerase chain reaction (PCR), Western blot, immunohistochemical staining. Pearson's correlation analysis was used to evaluate the between DYRK2. Transwell assay applied measure effect on migration invasion SW1116 SW480. We dual luciferase reporter confirm targeted binding 3'-untranslated region (3'UTR). An antisense experiment executed further role had played with regard targeting pathway cells. Results: In our research, we found elevated tissues cell lines compared nonCRC normal human colon epithelial line NCM460. Correspondingly, showed contrary tendency. closely correlated clinicopathological characteristics patients. demonstrated down-regulation could inhibit ability Also, confirmed negatively regulated via specific site within 3'UTR. finally verified cells conducted 3'UTR defect plasmid transfection group lower cotransfection group. Conclusion: findings this study indicate decrease suppress miR-622/DYRK2 be potential molecular treating target CRC. Keywords: miR-622, DYRK2, migration, invasion,