<em>LEF1-AS1</em>, a long non-coding RNA, promotes malignancy in glioblastoma

• DOI: 10.2147/ott.s130365
• Publication Date: 2017-08-28T19:42:08Z
• Authors: Jin Wang; Xiaoyang Liu; Changsheng Yan; Jie Liu; Songtao Wang; Yongzhi Hong; Aihua Gu; Peng Zhao
• Keywords: 0301 basic medicine; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

Objectives: The long-noncoding RNAs (lncRNAs) are identified as new crucial regulators of diverse cellular processes in glioblastoma (GBM) tissues. However, the expression pattern and biological function lncRNAs remain largely unknown. Here, for first time, effects lncRNA lymphoid enhancer-binding factor 1 antisense RNA ( LEF1-AS1 ) on GBM progression both vitro vivo investigated. Materials methods: Expression profiles specimens were investigated by bioinformatics analyses. tissues was detected using a quantitative polymerase chain reaction. inhibited transfecting -specific small interfering (siRNAs) stable cell lines established si- viruses. Cell Counting Kit-8, ethynyl deoxyuridine, colony formation assay used to examine proliferation function. flow cytometry cell-cycle change apoptosis. Migration Transwell assay. tumor xenografts immunohistochemistry performed evaluate growth vivo. Results: In this study, found significantly upregulated compared with normal 5-year overall survival patients from Cancer Genome Atlas high inferior that low expression. It confirmed higher than ones. Knockdown malignancy cells, including invasion, promoted result Western blot assays indicated knockdown -mediated suppression cells may be via reduction ERK Akt/mTOR signaling activities. Finally, experiment also demonstrated growth-promoting effect LEF1-AS U87 cells. Conclusion: Our acts an oncogene pivotal target disease. Keywords: lncRNA, , glioblastoma, proliferation, apoptosis