MicroRNA-381 enhances radiosensitivity in esophageal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein

• DOI: 10.2147/ott.s134551
• Publication Date: 2017-05-11T23:38:55Z
• Authors: Suna Zhou; Yaoyou Cui; Dequan Yu; Jun Liang; Mingxin Zhang; Ye Wenguang
• Keywords: XIAP; Radioresistance; Radiosensitivity; Ectopic expression; Inhibitor of apoptosis

Background: Increasing evidence indicates that radioresistance remains a major problem in the treatment of patients with esophageal squamous cell carcinoma (ESCC). This study was designed to investigate expression microRNA-381 (miR-381) and its function ESCC. Methods: In this study, miR-381 first detected ESCC lines tissue samples by quantitative real-time polymerase chain reaction (qRT-PCR). Then, effects on growth, apoptosis, radiosensitivity cells were analyzed MTT, colony formation, flow cytometry, respectively. Dual-luciferase reporter assays performed validate regulation putative target miR-381, corroboration qRT-PCR Western blotting assays. Results: or tissues found express significantly lower than normal epithelial adjacent tissues, Ectopic blocked proliferation, reduced enhanced increased enhancing irradiation-induced apoptosis. addition, dual-luciferase showed binds 3'-untranslated region X-linked inhibitor apoptosis protein (XIAP), suggesting XIAP should be direct miR-381. Re-expression suppressed cells, upregulation similar silencing XIAP. mRNA inversely correlated expression. Conclusion: The results suggest miR-381/XIAP pathway contributed growth inhibition, increase enhancement Therefore, may potential therapeutic human Keywords: carcinoma, XIAP,