Silencing of <em>c-jun</em> decreases cell migration, invasion, and EMT in radioresistant human nasopharyngeal carcinoma cell line CNE-2R

• DOI: 10.2147/ott.s162700
• Publication Date: 2018-07-03T22:58:30Z
• Authors: Guoxiang Lin; Binbin Yu; Zhongguo Liang; Ling Li; Song Qu; Kaihua Chen; Lei Zhou; Qiteng Lu; Yongchu Sun; Xiaodong Zhu
• Keywords: c-jun

Background: Previously, we found that c-jun was highly expressed in radiation-resistant human nasopharyngeal carcinoma cells (CNE-2R) compared with (CNE-2). Materials and methods: In this study, first used the scratch assays transwell to detect migration invasion of CNE-2R CNE-2 tested epithelial mesenchymal transformation (EMT)-related proteins E-cadherin N-cadherin by Western blot analysis. Subsequently, knocked down establish effect on EMT, migration, both vitro vivo. Results: A high EMT level, were more capable than cells. Moreover, silencing has upregulated expression downregulated cells, subsequently capacity decreased. Consistent results, vivo studies indicated reduced pulmonary Immunohistochemistry lung metastatic tumor tissue showed upregulated, downregulated. Conclusion: These findings suggest reduces invasion, Keywords: carcinoma, ,