Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells

• DOI: 10.2147/ott.s167187
• Publication Date: 2018-10-04T22:02:02Z
• Authors: Jinli Liu; Yuanyuan Guo; Yuanyuan Huang; Haowei Xue; Suwen Bai; Jinhang Zhu; Xianming Xia; Bing Shen; Wei Fang
• Keywords: IGFBP3; A431 cells; Epidermoid carcinoma

Cutaneous squamous cell carcinoma (CSCC) is the second most common worldwide. Clinical treatment for patients with CSCC remains non-ideal. Insulin-like growth factor binding protein 3 (IGFBP3), a member of insulin-like (IGF) system, participates in several biological processes, including cellular proliferation and apoptosis. Here, we explored functional role IGFBP3 apoptosis A431 cells, human line.Differential expression analysis, immunohistochemistry, immunoblotting, TUNEL assay, CCK8 assay techniques were used to investigate levels both cells tissue surgically obtained from humans as well explore cells.By using normal epidermal keratinocytes comparison, identified top 10 ranked differentially upregulated genes expressed cutaneous lines. Among these genes, was number 1. By found that level significantly elevated compared skin tissue. Knockdown by transfection IGFBP3-specific siRNA markedly altered proteins contribute via mitochondrial pathways, suppressing Bax active caspase-3, while increasing B-cell lymphoma-2 expression. confirmed effect knockdown on well. In addition, inhibited cells.IGFBP3 overexpressed lines enhanced pathway cells. These findings indicate may be biomarker potential therapeutic target CSCC.