GDF11 restrains tumor growth by promoting apoptosis in pancreatic cancer

• DOI: 10.2147/ott.s181792
• Publication Date: 2018-11-26T23:18:44Z
• Authors: Yanzhe Liu; Lijuan Shao; Kuang Chen; Zizheng Wang; Jin Wang; Wei Jing; Minggen Hu
• Keywords: 0301 basic medicine; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

GDF11 restrains tumor growth by promoting apoptosis in pancreatic cancer Yanzhe Liu,1,* Lijuan Shao,2,* Kuang Chen,1 Zizheng Wang,1 Jin Wei Jing,3 Minggen Hu1 1Department of Hepatobiliary and Pancreatic Surgical Oncology, Chinese PLA General Hospital, Beijing, People's Republic China; 2Translational Medicine Collaborative Innovation Center, The Second Clinical Medical College (Shenzhen Hospital), Jinan University, Shenzhen, 3Department Surgery, Changhai Military Shanghai, China *These authors contributed equally to this work Background: Growth differentiation factor (GDF) acted as a that regulated proliferation, several tumors. However, the effects (GDF11) remain unclear.Purpose: To investigate expression significance cancer.Patients methods: corresponding paracancerous tissues (n=28) were collected from Department Oncology Hospital. Tissue microarray was obtained Outdo Biotech Co., Ltd. (Shanghai, China). mRNA protein expressions samples cell lines detected using qRT-PCR, Western-Blot immunohistochemistry. Overexpression knockdown performed with lentiviral transduction system siRNA technique PANC-1 line CFPAC-1 line. Proliferation, migration invasion examinated MTS transwell assay, respectively. Flow cytometry used for analysis.Results: results study indicated significantly down-regulated compared adjacent cancer. also associated low when normal In cohort 63 patients, high levels favorable perineural invasion, T classification, N classification overall survival (OS). Cox proportional hazards model revealed an independent predictor prognosis (HR: 0.496; 95% CI: 0.255–0.967; P=0.040). cells repressed abilities vitro. Inhibition showed inverse results. Furthermore, enhanced promoted inhibited lines. Conclusion: These findings suggested suppressor gene Keywords: GDF11, cancer, apoptosis,