<p>Effect of Th9/IL-9 on the growth of gastric cancer in nude mice</p>

• DOI: 10.2147/ott.s197816
• Publication Date: 2019-03-25T19:12:07Z
• Authors: Li Cai; Yue Zhang; Yifei Zhang; Hongbing Chen; Jinchen Hu
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; 0302 clinical medicine; OncoTargets and Therapy; Original Research; 3. Good health

By neutralizing IL-9 in a nude mouse model, the study aimed to investigate role of Th9/IL-9 on growth gastric cancer mice.Male BALB/c mice were randomly divided into three groups: normal control group (Control), an SGC-7901 xenografted model (Model), and rIL-9 treatment (Treat). The weight tumors was recorded calculate tumor inhibition rate. Flow cytometry used detect cell frequency Th9, Th17, Treg peripheral blood. IL-4, IL-9, IL-10, IL-25, VEGF, TGF-β levels serum determined by ELISA. cellular migration invasion investigated transwell assay. Immunohistochemical Western blot expression CD34, PU.1, p53, p21 proteins tissue. mRNA IL-21, PU.1 tissue qRT-PCR.rIL-9 can significantly inhibit cancer. blood decreased upon treatment. reduced Treat compared with Model (P<0.05). reduce level PU.1. Meanwhile, group, reduced, whereas p53 increased (P<0.05).This suggested that has deleterious