<p>Long Non-Coding RNA TTN-AS1 Promotes the Proliferation and Invasion of Colorectal Cancer Cells by Activating miR-497-Mediated PI3K/Akt/mTOR Signaling</p>

• DOI: 10.2147/ott.s229104
• Publication Date: 2019-12-26T22:23:51Z
• Authors: Zhenhua Cui; Bingbing Han; Xianren Wang; Zhiwen Li; Jianxin Wang; Yanfeng Lv
• Keywords: 0303 health sciences; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

Introduction: Long non-coding RNAs (lncRNAs) have obtained increasing attention due to their regulatory functions in many cancers. This work aimed investigate the functional roles of lncRNA TTN-AS1 colorectal cancer (CRC) and explore underlying mechanisms. Methods: The expression profiles miR-497 CRC tissues cell lines were determined by RT-qPCR analysis. MTT assay, transwell western blot analysis, xenograft tumors nude mice employed analyze effects on proliferation, migration, invasion, EMT, vivo tumorigenesis cells. Bioinformatics analysis dual-luciferase reporter assay direct binding relation between CRC. Results: We observed a significant increase level compared with normal counterparts. High predicted poor prognosis was correlated aggressive clinicopathological characteristics patients. Functionally, gain- loss-of-function studies indicated that knockdown suppressed invasion epithelial–mesenchymal transition cells vitro, whereas overexpression showed complete opposite effects. Mechanistically, we found could directly interact miR-497, co-transfection mimics blocked activation PI3K/Akt/mTOR signaling, reversed Conclusion: To conclude, our findings provide novel insight into indicate may serve as potential therapeutic target for treatment. Keywords: cancer, long RNA TTN-AS1, epithelial-mesenchymal transition, microRNA-497, signaling