<p>Novel Alternatively Spliced Variants of Smad4 Expressed in TGF-β-Induced EMT Regulating Proliferation and Migration of A549 Cells</p>

• DOI: 10.2147/ott.s247015
• Publication Date: 2020-03-10T18:30:03Z
• Authors: Rongxue Wan; Xichao Xu; Lunkun Ma; Ying Chen; Liling Tang; Jianguo Feng
• Keywords: 0301 basic medicine; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

Introduction: Non-small cell lung cancer (NSCLC) is a worldwide malignance threatening human life. TGF-β/Smad signaling known to regulate proliferation, differentiation, migration and growth. As the only co-Smad playing crucial roles in TGF-β signaling, Smad4 reported be frequently mutated or occur as alternatively spliced tumor cells. was involved TGF-β-induced EMT process. However, whether alternative splicing occurs process NSCLC not clear. Methods: In our current study, we explored of during A549 10 ng/mL used induce EMT. Then, nest-PCR agarose electrophoresis were performed detect expression variants sequencing get variant DNA sequences. For recombinant cells, lentiviral infect order explore effects on proliferation MTT assay, colony formation assay wound-healing done. The E-cad VIM protein through Western blot. Results: There several novel gene fragments expressed results showed that they indeed reported. found have significant also regulated expression. Conclusion: Our indicated functional study these affect expression, showing potential targets for therapy. Keywords: Smad4, EMT, TGF-β, variants, non-small