<p>Radiotherapy Increases 12-LOX and CCL5 Levels in Esophageal Cancer Cells and Promotes Cancer Metastasis via THP-1-Derived Macrophages</p>

• DOI: 10.2147/ott.s257852
• Publication Date: 2020-08-04T20:49:45Z
• Authors: Si Mi; Yan Qu; Xue Chen; Zhihua Wen; Pengxiang Chen; Yufeng Cheng
• Keywords: CCL5

Dioxygenase 12-lipoxygenase (12-LOX) plays an important role in tumorigenesis and promotes angiogenesis proliferation several tumors, including prostate breast tumors. Radiotherapy enhances the expression of 12-LOX esophageal squamous cell carcinoma (ESCC). Two types macrophages can be found tumor microenvironment. The M2 subtype accelerates progression; however, relationship between is not well established. Here, we explore this interaction its effect on ESCC to induce progression.RT-qPCR Western blot analyses were used evaluate mRNA protein levels chemokine (C-C motif) ligand 5 (CCL5) after radiotherapy. CCL5 was increased by upregulation but suppressed well-established inhibitor, baicalein. Furthermore, attracted repolarized human myeloid leukemia mononuclear cells (THP-1)-derived macrophages. Finally, co-culture with THP-1-derived led a strong cancer migratory capacity.Radiation-induced overexpression upregulates expression, thereby attracting promoting their polarization subtype, which cellular metastasis.