<p>Effect of <em>Malus asiatica</em> Nakai Leaf Flavonoids on the Prevention of Esophageal Cancer in C57BL/6J Mice by Regulating the IL-17 Signaling Pathway</p>

• DOI: 10.2147/ott.s261033
• Publication Date: 2020-07-16T20:22:48Z
• Authors: Xiang-Bo Jia; Quan Zhang; Lei Xu; Wen-Jian Yao; Li Wei
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

The aim of this study was to observe the preventive effect flavonoids extracted from Malus asiatica Nakai leaves (FMANL) on esophageal cancer in mice, especially ability FMANL regulate interleukin 17 (IL-17) signaling pathway during process.The C57BL/6J mice were treated with 4-nitroquinoline N-oxide (4NQO) induce cancer, and visceral tissue index serum indexes used verify FMANL.The experimental results showed that can effectively control changes caused by cancer. could increase cytokine levels 10 (IL-10), monocyte chemotactic protein 1 (MCP-1) decrease tumor necrosis factor alpha (TNF-α), interferon-γ (IFN-γ), 6 (IL-6), 12p70 (IL-12p70) also reduce CD3+, CD4+, CD8+ enhance CD19+ mouse peripheral blood lymphocytes. qPCR Western blot analysis down-regulate mRNA expression IL-17, 23 (IL-23), beta (IL-1β), chemokine (C-X-C) ligand (CXCL1), 2 (CXCL2), S100 calcium-binding A8 (S100A8), A9 (S100A9), matrix metalloprotein 9 (MMP-9), 13 (MMP-1) High-performance liquid chromatography (HPLC) detection contained chemicals, including rutin, hyperoside, isoquercitrin, dihydroquercetin, quercitrin, hesperidin, myricetin, baicalin, neohesperidin dihydrochalcone, quercetin.It be concluded prevent experimentally induced its effects might obtained compounds present FMANL.