<p>Knockdown of LncRNA DLEU2 Inhibits Cervical Cancer Progression via Targeting miR-128-3p</p>

• DOI: 10.2147/ott.s272292
• Publication Date: 2020-10-08T23:56:50Z
• Authors: Bofei Wang; Jing Hang; Weiling Li; Wanqiong Yuan
• Keywords: 0301 basic medicine; 03 medical and health sciences; OncoTargets and Therapy; Original Research; 3. Good health

Cervical cancer is one of the most common female malignancies worldwide and represents a major global health challenge. The fast growth tumor high rates metastasis still lead to poor prognosis cervical patients. It urgent clarify mechanism identify predictive biomarkers for treatment cancer. Long non-coding RNAs (LncRNAs) have been identified in are related malignant phenotypes cells. However, roles LncRNA deleted lymphocytic leukemia (DLEU2) tumorigenesis progression remain unknown.qPCR was performed analyze expression DLEU2, Cyclin D1, CDK4, Bax, Bcl2 mi-128-3p. Western blot detect cell cycle hallmarks expression. CCK8 used examine proliferation. Cellular apoptosis analyzed by Hoechst 33,258 staining AV/PI with flow cytometry. Cell xenograft model nude mice elucidate function DLEU2 vivo. Bioinformatics analysis luciferase reporter assay were proceeded whether miR-128-3p directly binds lncRNA DLEU2. Pull‑down RNA-binding protein immunoprecipitation exploring relationship between miR-128-3p.We demonstrated that upregulated tissues. Downregulation inhibited proliferation, induced arrest at G2/M phase cells vitro, suppressed Further, targets miR-128-3p. inhibitor abrogated proliferation knockdown reversed regulated DLEU2.Taken together, these results suggested via targeting