<p>Long Non-Coding RNA LINC00662 Regulated Proliferation and Migration by Targeting miR-34a-5p/LMAN2L Axis in Glioma</p>

• DOI: 10.2147/ott.s272616
• Publication Date: 2020-10-08T23:56:56Z
• Authors: Yibo Geng; Yuliang Wu; Cheng Xu; Tian Li; Liwei Zhang
• Keywords: Competing Endogenous RNA

Numerous studies suggest that long non-coding RNAs (lncRNAs) participate in the biological process of diverse malignancies, including glioma. Although many differentially expressed lncRNAs have been identified glioma, to our best knowledge, role LINC00662 and its potential underlying mechanism glioma progression remains unclear. This study aimed explore function regulatory network glioma.Expressions LINC00662, miR-34a-5p lectin mannose-binding 2-like (LMAN2L) tissues were analyzed using The Cancer Genome Atlas Program (TCGA) Chinese Glioma (CGGA) databases. Colony formation, Celltiter-Glo BrdU (5-bromo-2'-deoxyuridine) incorporation assays used detect cell proliferation vitro. Xenograft mouse models established determine vivo. Transwell wound healing assay was migration. In addition, epithelial-mesenchymal transition (EMT) markers detected by Western blot. Annexin V 7-AAD stain apoptotic cells. Interactions between or 3'-UTR LMAN2L predicted determined bioinformatics analysis, luciferase reporter RNA immunoprecipitation (RIP) assays.High level poor overall survival patients. Functional revealed suppression remarkably inhibited proliferation, clonogenicity EMT pathway. Mechanistically, sponged regulate expression. Furthermore, inhibitor reversed anti-proliferation anti-migration effect knockdown, which could be rescued downregulation cells.Our first report acted as a competing endogenous (ceRNA) targeting miR-34a-5p/LMAN2L axis, providing new therapeutic target for