<p>Profiling and Integrated Analysis of Differentially Expressed Circular RNAs in Plasma Exosomes as Novel Biomarkers for Advanced-Stage Lung Adenocarcinoma</p>

• DOI: 10.2147/ott.s279710
• Publication Date: 2020-12-17T21:40:31Z
• Authors: Shan Lin; Wenji Xiong; Huibo Liu; Liping Pei; Huanfa Yi; Yinghui Guan
• Keywords: Profiling (computer programming)

Purpose: Exosomes contain abundant circRNAs and are determined to be involved in the pathogenesis of lung adenocarcinoma (LUAD). Thus, our study aimed explore new plasma exosomes that could such pathogenesis. Patients Methods: High-throughput sequencing was used identifying alterations exosomal circRNA expression. Gene ontology functional analysis (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway were performed determine significant functions pathways associated with differentially expressed circRNAs. TargetScan miRanda predict circRNA-targeted microRNAs mRNAs. CircRNA expression profiles then validated by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Wound healing Transwell assays roles has_circ_0102537 LUAD progression. Results: We identified six significantly upregulated 214 downregulated GO KEGG suggested occurrence development LUAD. A circRNA–miRNA–mRNA meshwork established potential interactions among these RNAs. The profile subjected qRT-PCR for validation. hsa_circ_0102537 both tissues. GO, analysis, meshwork, further experiments suggest Conclusion: Our explored a large number may pathogenesis, thereby supporting need research on diagnosis biomarkers intervention therapeutic targets. Keywords: advanced-stage adenocarcinoma, plasma, exosomes, circular RNA, microRNA