miR-21 Induces Chemoresistance in Ovarian Cancer Cells via Mediating the Expression and Interaction of CD44v6 and P-gp

• DOI: 10.2147/ott.s286639
• Publication Date: 2021-01-12T20:21:44Z
• Authors: Yanqing Wang; Gantao Chen; Fangfang Dai; Li Zhang; Mengqin Yuan; Dongyong Yang; Shiyi Liu; Yanxiang Cheng
• Keywords: 03 medical and health sciences; 0302 clinical medicine; OncoTargets and Therapy; Original Research; 3. Good health

Background: Ovarian cancer (OC), a representative female reproductive system tumor, is one of the most malignant tumors in female. The important reason for its poor prognosis because high rate chemotherapy resistance. Results: This study aims to explore effects miR-21 on resistance OC cells. functions proliferation, migration and invasion cells were assessed by transwell, clonal formation CCK8 assay. Expression levels miR-21, P-gp CD44v6 SKOV3 (cisplatin sensitive) SKOV3/DDP resistant) detected quantitative reverse transcription-polymerase chain reaction (qRT-PCR) Western blotting. Si-CD44v6 was transfected into detect influence P-glycoprotein (P-gp) expression. Immunofluorescence used localization cell. Co-immunoprecipitation relationship between P-gp. Results showed that expression cisplatin-resistant significantly higher than cells, at same time, as well ability enhanced after mimics cisplatin-sensitive Furthermore, level affected co-immunoprecipitation protein could interact. Conclusion: In conclusion, increase invasion, And interaction may mediate involvement Keywords: ovarian cancer, resistance, CD44v6,