BIOLOGICAL CHANGES OCCURED IN SOYBEAN SEED DURING EXPOSING TO SEVERAL TYPES OF SEED PRIMING

• DOI: 10.21608/ajs.2018.31654
• Publication Date: 2019-06-10T06:08:54Z
• Authors: H.M. Mohamed; A.M., Zaki; Olfat H. El-Bagoury; Rania A.A. Younis
• Keywords: Priming (agriculture); Dry weight; Seed treatment

Biological experiments were carried out at Agronomy Seed Lab., Dept., Faculty of Agriculture, Ain Shams University and Center For Genetic Engineering Biotechnology ACGEB, during 2016/2017 season. Priming its duration investigated whereas different types priming (hydro-priming, osmopriming salt-priming) periods each type (short- medium long periods) stuied. Newly harvested soybean seeds cultivar (Giza 111) submitted from Field Crop Institute, Agricultural Research (ARC). It was found that enhanced germination percentage significantly low performance 40% to 51%, 68% 75.5% for hydration, salt respectively. Extending exposing period the longest gave maximum increment in seed germination. Maximum enhancement achieved when calcium chloride solution used (48 hrs.) giving value 96.0%. Such reached 140% as compared with control. noticeable increasing soaking examined this investigation accelerated rate a level. Salt produced seedling shoot Overall, most results obtained trial, primed CaCl2 showed better perfor mance than those water or PEG solution. Seedling dry weight revealed significant effect similar manner length. The on weight. Soybeans proteins exposed all three varied control, number protein bands SDS gel increased 10 separated control pattern 13, 11 12 hydration treatment 6, 24 hrs., Also mono poly-uni. unique well intensive dye which reflect amount formed between treatments. Considering less gel. quality assessed by extracting native polyacrylamide electrophoresis, whereas, application caused peroxidase activity (untreated seeds). remarkably expose highest compare also short (6 hrs.). it noticed there diffuse band end lane, these staining color, meaning their activities is extracted hydro-primed hrs. respectively). esterase had two prolonged regions, appeared bands. Esterase PAGE subjected any (long, short) untreated (control). clear reduced since diminished color.