HISTOPATHOLOGICAL AND BIOCHEMICAL ASSESSMENT OF LIVER FIBROSIS INDUCED BY CARBON TETRACHLORIDE ADMINISTARTION IN RAT

• DOI: 10.21608/avmj.2023.181348.1111
• Publication Date: 2023-04-24T11:18:56Z
• Authors: EMAN SHAABAN; NASHWA HAMAD; DENA TORRA; MOKHTAR TAHA
• Keywords: Histopathology; CCL4; Histopathological examination

This study investigated liver fibrosis and hepatotoxicity caused by carbon tetrachloride (CCL4) in albino rats.Two groups of twenty-two adult male rats, each weighing 150 to 170 gms, were created.Ten rats from the CCL4-administered group (I) received subcutaneous injections CCL4 olive oil at a dose 2 ml/kg twice weekly for 12 consecutive weeks.The control (II) contained twelve was divided into two six each.One served as standard control, other only got via same route dosage (I).All sacrificed weeks post dosing, tissue specimens livers collected histopathological examination.Additionally, serum samples order measure various biochemical factors, including (alanine aminotransferase (ALT), aspartate (AST), total protein (TP), antioxidant capacity (TAC) lipid peroxidation (LPO).The examination I showed hepatic degenerative, necrotic angiopathic alterations, well pronounced fibrosis.The II normal appearance with no pathological changes.The results detected significant upregulation AST, ALT LPO compared group, while TP TAC significantly downregulated.In conclusion, administered variable degenerative changes, besides activation proliferative potential collagen fibers, changed parameters rats.