In vitro Antigen-presentation Assay for Self- and Microbial-derived Antigens

• DOI: 10.21769/bioprotoc.2307
• Publication Date: 2017-06-06T07:21:07Z
• Authors: Laura Campisi
• Keywords: Antigen processing

Antigen presenting cells (APC) are able to process and present T antigens from different origins.This mechanism is highly regulated, in particular by Patter Recognition Receptor (PRR) signals.Here, I detail a protocol designed assess vitro the capacity of APC derived bacteria, apoptotic infected cells.