Presence of the Transmembrane Protein Neuropilin in Cytokine-induced Killer Cells

• DOI: 10.21873/anticanres.14560
• Publication Date: 2020-09-29T00:40:46Z
• Authors: EUGENIA V. DÍEZ G...; FRANCESCA GAROFANO; HANS WEIHER; MICHAEL MUDERS; SARAH FÖRSTER; INGO G.H. SCHMIDT...
• Keywords: Cytokine-induced killer cell

Background/Aim: Cytokine-induced killer (CIK) cells are a heterogenous population of immune showing promising applications in immunotherapeutic cancer treatment. Neuropilin (NRP) proteins have been proven to play an important role development and prognosis. In this study, CIK were tested for expression NRPs, transmembrane playing the proliferation survival cells. Materials Methods: analyzed at different time points via flow cytometry quantitative real-time polymerase chain reaction neuropilin expression. Results: Phenotyping results showed having developed properly, low levels NRP2 detected. On other hand, no NRP1 was found. Two cell lines by cytometry: A549 expressed NRP2; U251-MG high amounts NRP2. on day 14. Conclusion: The presence NRP2, but not NRP1, shown Recognizing might help improve cytotoxicity.