Genetic Diversity of Plasmodium falciparum Parasites in Pregnant and Non-pregnant Women and Potential Resistance to Antimalarial Drugs in Kenya

• DOI: 10.21955/aasopenres.1114904.1
• Publication Date: 2019-08-05
• Authors: Brenda Makena; Victor A. Mobegi; Martha Nginya; Gladys Chemwor; Raphael Okoth; Benjamin Opot; Dennis W. Juma; Hoseah M. Akala; Ben Andagalu; Jim Ray Managbanag
• Keywords: Clearance; Parasite load

Non-pregnant women clear parasites faster than pregnant. Non-immune environment in pregnant may select for parasite populations associated with artemisinin resistance. Objective: To identify antimalarial drugs resistance polymorphisms expressed P. falciparum versus non-pregnant Kenya. Methods Blood samples were collected at hours 0, 8, 24; days 7 and 28 from 75 malaria positive women. Arm1(pregnant second trimester), arm2(pregnant third trimester) Arm3(non-pregnant). Samples diagnosed using rtPCR. Merozoite surface proteins 1 2 genotyping done to determine recrudescence reinfection. Single nucleotide genotyping genes linked population structure determination proceeded. Results 40.0% of the 15 participants arm1, 16.7% 12 arm2 5.6% 18 arm3 participants were Plasmodium positive Hr24. At day7, however, 66.7% arm1 still while 3 had both cleared parasite. All P. falciparum infections after 24hrs treatment. 225 375 samples proceeded subsequent analysis. Conclusion positives on confirmed non-falciparum reinfections. This study presents genotypic properties advises on expression between three arms.