Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility.

• DOI: 10.22074/cellj.2022.557547.1065
• Publication Date: 2023-01-01
• Authors: Shima Abbasihormozi; Azam Kouhkan; Abdolhossein Shav...; Mohammad Ali Sadi...; Vahab Babapour; Amir Niasari Naslji; Vahid Akbarinehad; AliReza Alizadeh
• Keywords: Pathogenesis

Although the role of obesity and diabetes mellitus (DM) in male infertility is well established, little information about underlying cellular mechanisms available. In this sense, nuclear factor kappa-B (NF-kB) has been recognized as an important regulator DM; However, its function pathogenesis never studied obese or men who suffer from diabetes. Therefore, main goal current research assessing NF-kB existence activity ejaculated human spermatozoa considering diabetics condition males.In experimental study, ELISA technique was applied to analyze levels sperm four groups: non-obese none-diabetic (body mass index (BMI) <25 kg/m2; control group; n=30), non-diabetic (BMI >30 OB diabetic DM OB-DM n=30) were presented Royan Institute Infertility Center. addition, protein localization shown by Immunocytofluorescent assay. Sperm features also evaluated using CASA.The older than regardless status (P=0.0002). progressive motility affected (P=0.035) type A (P=0.034). The concentration (P=0.013), (P=0.025) morphology (P<0.0001) altered × interaction effects. negatively influenced impact (P=0.019). Obesity did not affect (P=0.248) activity. Uniquely, localized midpiece post-acrosomal areas.The study indicated a lower men, no effect on observed yet. Additionally, we revealed effects, their adversely characteristics.