Effects of propolis supplementation during cryopreservation of ram semen.

• DOI: 10.22099/ijvr.2024.49410.7253
• Publication Date: 2024-01-01
• Authors: İbrahim Halil Güngör; Şeyma Özer Kaya; Serap Dayan Cinkara; A Çakır Cihangiroğlu; Emre Kaya; Tutku Can Acısu; Figen Erdem Erişir; Ömer Yılmaz; Seval Yılmaz; Seyfettin Gür; Mustafa Sönmez; Gaffari Türk
• Keywords: Propolis; Semen cryopreservation

Cryopreservation of ram semen is a very challenging process. Loss motility during freezing does not allow artificial insemination rams. This study aimed to determine whether the inclusion liquid propolis extract in diluents affects cryopreservation efficiency semen. Six Akkaraman breed rams were considered for study. Semens combined with Tris+egg yolk extender containing and without (control) at different concentrations (0.25%, 0.50%, 1%, 2%, 4%). Semen was frozen using routine procedures. After thawing, kinematic parameters analyzed by computer assisted analysis (CASA), viability, acrosomal damage level mitochondrial membrane potential flow-cytometer all groups. Additionally, fatty acid levels total gas chromatography (GC), vitamin cholesterol high-performance (HPLC). In addition, oxidative stress, HOS test, morphological analyzes performed after thawing. The 0.5% group showed significant increase rapid motility, LIN, integrity, antioxidant compared control, decrease malondialdehyde (MDA) low (LMMP) levels. Compared 4% damaged spermatozoa caused total, progressive high (HMMP), Glutathione S-transferase (GST), catalase (CAT) We that adding extenders freezability increases survival freeze-thaw ensures success freezing.