Blood Microbiome Profile in CKD

• DOI: 10.2215/cjn.12161018
• Publication Date: 2019-04-08T17:47:07Z
• Authors: Neal B. Shah; Andrew S. Allegretti; Sagar U. Nigwekar; Sahir Kalim; Sophia Zhao; Benjamin Lelouvier; Florence Servant; Gloria Serena; Ravi Ishwar Thadhani; Dominic S. Raj; Alessio Fasano
• Keywords: Dysbiosis

The association between gut dysbiosis, high intestinal permeability, and endotoxemia-mediated inflammation is well established in CKD. However, changes the circulating microbiome patients with CKD have not been studied. In this pilot study, we compare blood profile healthy controls using 16S ribosomal DNA sequencing.Blood bacterial was studied buffy coat samples quantitatively by PCR qualitatively targeted metagenomic sequencing a molecular pipeline specifically optimized for cross-sectional study comparing 20 nondiabetic controls.There were 22 operational taxonomic units significantly different two groups. revealed significant reduction α diversity (Chao1 index) group compared (127±18 versus 145±31; P=0.04). Proteobacteria phylum, Gammaproteobacteria class, Enterobacteriaceae Pseudomonadaceae families more abundant controls. Median levels did differ groups (117 122 copies/ng DNA; P=0.38). GFR correlated inversely proportion of (r=-0.54; P≤0.01).Our demonstrates qualitative differences lower variations