DNA Methylation and Soy Phytoestrogens: Quantitative Study in DU-145 and PC-3 Human Prostate Cancer Cell Lines

• DOI: 10.2217/epi.11.103
• Publication Date: 2011-11-25T15:21:03Z
• Authors: Mawussi Adjakly; Rémy Bosviel; Nadège Rabiau; Jean-Paul Boiteux; Yves-Jean Bignon; Laurent Guy; Dominique Bernard...
• Keywords: BRCA2 Protein; Male; 2. Zero hunger; Glycine max; BRCA1 Protein; Receptor, EphB2; Blotting, Western; Prostatic Neoplasms; Phytoestrogens; DNA Methylation; Flow Cytometry; Genistein; Isoflavones; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Glutathione S-Transferase pi; Cell Line, Tumor; Humans; Genes, Tumor Suppressor; Promoter Regions, Genetic

Aim: DNA hypermethylation is an epigenetic mechanism which induces silencing of tumor-suppressor genes in prostate cancer. Many studies have reported that specific components food plants like soy phytoestrogens may protective effects against carcinogenesis or progression. Genistein and daidzein, the major phytoestrogens, been to ability reverse cancer cell lines. The aim this study was investigate potential demethylating these two compounds on BRCA1, GSTP1, EPHB2 BRCA2 promoter genes. Methods & materials: Prostate lines DU-145 PC-3 were treated with genistein 40 µM, daidzein 110 budesonide (methylating agent) 2 µM 5-azacytidine (demethylating µM. In human we performed methylation quantification by using Methyl Profiler analysis. This technique based a methylation-specific digestion followed quantitative PCR. We analyzed corresponding protein expression western blotting. Results: Soy induced demethylation all regions studied except for BRCA2, not methylated control An increase their also demonstrated blot analysis corroborated effect phytoestrogens. Conclusion: showed induce decrease GSTP1 promoters. Therefore, However, more are needed order understand inhibiting action methylation.