EndoSd: An IgG Glycan Hydrolyzing Enzyme in Streptococcus Dysgalactiae Subspecies Dysgalactiae

• DOI: 10.2217/fmb.16.14
• Publication Date: 2016-05-21T20:05:12Z
• Authors: Azadeh Shadnezhad; Andreas Naegeli; Jonathan Sjögren; Barbara Adamczyk; Fredrik Leo; Maria Allhorn; Niclas G Karlsson; Anders Jensen; Mattias Collin
• Keywords: 0303 health sciences; IgG; Hydrolysis; Streptococcus; Disaccharides; Substrate Specificity; 03 medical and health sciences; Endo-β-N-acetylglucosaminidase; Bacterial Proteins; Polysaccharides; Immunoglobulin G; asparagine linked; glycosidase; Acetylglucosaminidase; chitinases; Animals; Humans; Streptococcus dysgalactiae; Phylogeny

The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD).PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd.EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium.Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.