Beta-2 Microglobulin Knockout K562 Cell-Based Artificial Antigen Presenting Cells For Ex Vivo Expansion of T Lymphocytes

• DOI: 10.2217/imt-2018-0211
• Publication Date: 2019-06-20T10:43:48Z
• Authors: Shijun Zha; Zhendong Li; Can Chen; Zhicheng Du; Johan Chin-Kang Tay; Shu Wang
• Keywords: K562 cells

Aim: The human K562 leukemia cell line as a scaffold of artificial antigen presenting cells (aAPCs) for ex vivo lymphocyte expansion does not usually express major histocompatibility complex (MHC) molecules. However, when stimulated by supernatants from T cultures, upregulate β-2 microglobulin (B2M) and MHC class I expression, which would induce allo-specific cells. Methods: We disrupted the B2M locus in CRISPR/Cas9 achieved I-negative Results: further generated K562-based aAPC zinc-finger nuclease mediated insertion costimulatory molecules into AAVS1 locus. This could attenuate allogeneic immune responses but support robust antigen-independent CD19 antigen-specific chimeric receptor-T vitro. Conclusion: B2M-knockout provide new construction broader application adoptive immunotherapies.