Selective Measurement and Manipulation of Adhesion Forces Between Cancer Cells and Bone Marrow Endothelial Cells Using Atomic Force Microscopy

• DOI: 10.2217/nnm.12.139
• Publication Date: 2012-12-02T13:13:48Z
• Authors: Kimberley J Reeves; Jia Hou; Susan E Higham; Zhe Sun; Jerome P Trzeciak...; Gerald A Meininger; Nicola J Brown
• Keywords: Force Spectroscopy

Aims: The lack of understanding the biology bone cancer metastasis has limited development effective treatment strategies. aim this study was to characterize tumor cell adhesion molecules and determine active interactions with human marrow endothelial (BME) cells using atomic force microscopy. Materials & methods: A single prostate (PC3) coupled (concanavalin A) microscopy cantilever then placed in contact BME for spectroscopy measurements. Results discussion: Strong adhesive between PC3 were significantly (p < 0.05) reduced by anti-ICAM-1, anti-β1 anti-P-selectin, but not anti-VCAM-1. combined blocking antibodies or therapeutic agent zoledronic acid 0.005) 65 63%, respectively, which confirmed a functional vitro assay. Conclusion: Atomic provides highly sensitive screening assay quantify nanoscale events different types important metastatic cascade. Original submitted 21 February 2012; Revised 30 July Published online 2 December 2012