CEA-targeted Nanoparticles allow Specific in Vivo Fluorescent Imaging of Colorectal Cancer Models

• DOI: 10.2217/nnm.14.202
• Publication Date: 2015-02-19T11:56:11Z
• Authors: James P Tiernan; Nicola Ingram; Gemma Marston; Sarah L Perry; Jo V Rushworth; P Louise Coletta; Paul A Millner; David G Jayne; Thomas A Hughes
• Keywords: Carcinoembryonic antigen; Fluorescence-lifetime imaging microscopy

Fluorescent imaging of colorectal tumor cells would improve localization and allow intra-operative staging, facilitating stratification surgical resections thereby improving patient outcomes. We aimed to develop test fluorescent nanoparticles capable allowing this in vivo. Dye-doped silica were synthesized. Anti-CEA (carcinoembryonic antigen) or control IgGs conjugated using various chemical strategies. Binding CEA-targeted cancer was quantified vitro, vivo after systemic-delivery murine xenografts. CEA-targeted, polyamidoamine dendrimer-conjugated, nanoparticles, but not allowed strong tumor-specific imaging. are the first demonstrate live, specific, antibody-targeted nanoparticles. These have potential visualization cells.