Bioinspired Negatively Charged Calcium Phosphate Nanocarriers for Cardiac Delivery of MicroRNAs

• DOI: 10.2217/nnm.16.26
• Publication Date: 2016-03-16T11:41:27Z
• Authors: Vittoria Di Mauro; Michele Iafisco; Nicolò Salvarani; Marco Vacchiano; Pierluigi Carullo; Gloria Belén Ramí...; Tatiana Patrício; Anna Tampieri; Michele Miragoli; Daniele Catalucci
• Keywords: cardiac disease; Calcium Phosphates; Biomedical Engineering; Medicine (miscellaneous); 610; cardiomyocyte; Bioengineering; cardiomyocytes; Biocompatible Materials; calcium phosphate nanoparticles; cardiomyocytes; drug-delivery systems; microRNA; nanomedicine; Animals; Biocompatible Materials; Calcium Phosphates; Cell Line; Cells, Cultured; Drug Carriers; Drug Delivery Systems; Gene Transfer Techniques; Humans; Mice, Inbred C57BL; MicroRNAs; Myocytes, Cardiac; Nanoparticles; Bioengineering; Medicine (miscellaneous); Biomedical Engineering; Materials Science (all); Cell Line; 03 medical and health sciences; drug-delivery system; Drug Delivery Systems; calcium phosphate nanoparticles; calcium phosphate nanoparticle; Animals; Humans; Myocytes, Cardiac; Development3304 Education; Nanoscience & Nanotechnology; Cells, Cultured; Cardiomyocytes; Drug Carriers; 0303 health sciences; 1007 Nanotechnology; drug-delivery systems; microRNA; 1004 Medical Biotechnology; nanoparticle; Gene Transfer Techniques; 540; nanomedicine; Drug-delivery; 3. Good health; Mice, Inbred C57BL; MicroRNAs; Nanomedicine; Calcium phosphate; Nanoparticles; Materials Science (all)

To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart.CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated.CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo.CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.