Perfluorodecalin and bone regeneration

• DOI: 10.22203/ecm.v025a02
• Publication Date: 2016-12-01T07:36:38Z
• Authors: F Tamimi; P Comeau; D Le Nihouannen; YL Zhang; DC Bassett; S Khalili; U Gbureck; SD Tran; S Komarova; JE Barralet
• Keywords: Calcium Phosphates; Male; 0301 basic medicine; bone marrow; Bone Regeneration; Cell Survival; Bone Marrow Cells; Diseases of the musculoskeletal system; bone; Mice; 03 medical and health sciences; bone regeneration; Bone Density; Materials Testing; Animals; radiopacity; Cells, Cultured; Bone Marrow Transplantation; Orthopedic surgery; Fluorocarbons; Mice, Inbred BALB C; Guided Tissue Regeneration; Hydrogen-Ion Concentration; Cell Hypoxia; Radiography; RC925-935; Perfluorocarbons; Bone Substitutes; Rabbits; Hydrophobic and Hydrophilic Interactions; RD701-811; biomaterials

Perfluorodecalin (PFD) is a chemically and biologically inert biomaterial and, as many perfluorocarbons, is also hydrophobic, radiopaque and has a high solute capacity for gases such as oxygen. In this article we have demonstrated, both in vitro and in vivo, that PFD may significantly enhance bone regeneration. Firstly, the potential benefit of PFD was demonstrated by prolonging the survival of bone marrow cells cultured in anaerobic conditions. These findings translated in vivo, where PFD incorporated into bone-marrow-loaded 3D-printed scaffolds substantially improved their capacity to regenerate bone. Secondly, in addition to biological applications, we have also shown that PFD improves the radiopacity of bone regeneration biomaterials, a key feature required for the visualisation of biomaterials during and after surgical implantation. Finally, we have shown how the extreme hydrophobicity of PFD enables the fabrication of highly cohesive self-setting injectable biomaterials for bone regeneration. In conclusion, perfluorocarbons would appear to be highly beneficial additives to a number of regenerative biomaterials, especially those for bone regeneration.