Osteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foams

• DOI: 10.22203/ecm.v043a10
• Publication Date: 2022-03-21T09:35:38Z
• Authors: J Luzuriaga; P García-Gallastegui; N García-Urkia; JR Pineda; I Irastorza; F-J Fernandez-San...; N Briz; B Olalde; F Unda; I Madarieta; G Ibarretxe
• Keywords: Osteopontin

3D cell culture systems based on biological scaffold materials obtainable from both animal and human tissues constitute very interesting tools for therapy personalised medicine applications. The white adipose tissue (AT) extracellular matrix (ECM) is a promising biomaterial engineering due to its easy accessibility, malleability proven activity. In the present study, dental pulp stem cells (hDPSCs) were combined in vitro with ECM scaffolds porcine decellularised (pDAT, hDAT) processed as solid foams, investigate their effects osteogenic differentiation capacity bone production of hDPSCs, compared single-protein-based foams collagen type I conventional 2D tissue-culture-treated polystyrene plates. pDAT supported hDPSCs similar levels I, assessed by alkaline phosphatase alizarin red stainings, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) osteocalcin/bone gamma-carboxyglutamate protein (BGLAP) immunostaining. Interestingly, hDAT showed markedly lower sustain hDPSC calcification higher support adipogenesis, RT-qPCR oil O staining. White ATs origins are relatively abundant available sources raw material obtain high quality ECM-derived biomedical products. These biomaterials could have applications clinical healing regeneration lesions involving not only loss calcified but also associated soft non-calcified tissues.