Genomic Determinants and Antimicrobial Resistance Pattern of Clinical Isolates of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli

• DOI: 10.22207/jpam.17.3.32
• Publication Date: 2023-09-01T17:42:06Z
• Authors: Hamid Ahmad Shah; Arshi Syed; Mohd Altaf Bhat; Dalip K. Kakru; Shaheen Farooq; Sabia Qureshi; Azhar Shafi; Burhan Nabi; Anil Taku
• Keywords: Beta-lactamase; Agar diffusion test

The growing prevalence of antibiotic-resistant bacteria is a worldwide public health apprehension, and Escherichia coli (E. coli) one the most commonly implicated bacterial species. Among E. isolates, extended-spectrum β-lactamase (ESBL)-producing strains have been identified as key contributor to antibiotic resistance. Penicillin’s, cephalosporins, monobactams are only few β-lactam antibiotics that can be rendered inactive by ESBLs. This investigation’s goals were determine ESBL-producing isolates found in clinical samples analyze distribution blaTEM, blaSHV, blaCTX-M genes among them. Additionally, we aimed susceptibility patterns these other antibiotics. Clinical from urine, ear swab, wound/pus swabs collected patients with suspected infections different regions north India viz., SKIMS-JVC medical college NABL accredited Dr. Qadri’s Lab both Srinagar Kashmir valley region SMSR, SU Greater Noida UP. Standard laboratory techniques used identify combined disc method phenotypic confirmation confirm ESBL formation. PCR amplification sequencing find genes. Kirby-Bauer diffusion was test antimicrobial various A total 210 158 showed positive results for DDST confirmatory tests. Of these, 124 (78.48%) isolates. We blaTEM prevalent gene (45.16%), followed (34.16%) blaSHV (12.09%). Antimicrobial resistance profiles assessed each 120 Ampicillin Cefepime resistant drugs Gentamicin, Ceftriaxone, Cefixime.