Isolation and Characterization of Bacteriocin Producing Levilactobacillus brevis Strain ABRIINW-K from Buffalo Dung

• DOI: 10.22207/jpam.18.3.64
• Publication Date: 2024-08-31T16:47:32Z
• Authors: Mohd Azam; Rashi Srivastava; Tanzeel Ahmed
• Keywords: Lactobacillus rhamnosus; Strain (injury)

Bacteriocins are proteins secreted by many species of bacteria to inhibit other bacteria, thus eliminating competitors gain resources. Bacteria from the Lactobacillus group known for their applications as probiotics and food preservatives. They have earned a reputation producing substances that growth microorganisms, which include organic acids, diacetyl, bacteriocins. Produced ribosomes, bacteriocins cationic coexisting within shared ecological habitat. Due potential uses in variety large-scale production would be necessary. The study aimed identify characterize produce potent analyze antimicrobial activity stability isolated bacteriocin under various physical biochemical conditions. A total 50 samples including buffalo dung, cheese, rhizospheric region plants were screened isolate 8 Li-1, Li-2, Li-3, Li-4, Li-5, Li-6, Li-7, Li-8, confirmed gram staining tests. cell free supernatant Li-3 strain showed higher inhibition Escherichia coli Staphylococcus aureus, compared strains. was further identified Levilactobacillus brevis ABRIINW-K 16S rRNA gene sequencing. this is thermostable peptide (~6kDa), characteristic class II bacteriocins, with antibacterial against rhamnosus, coli, Salmonella enterica.