Artificial infection with Fusarium solani f.sp. passiflorae in plants of passion fruit under controlled conditions

• DOI: 10.22267/rcia.20234003.219
• Publication Date: 2024-03-07T19:49:21Z
• Authors: Erika Patricia Ma...; Jairo Antonio Oso...; Juan Climaco Hio; Ginna Natalia Cru...; Jaime Esneider Ag...; Emerson Duvan Roj...; Johan Andrés Verg...
• Keywords: Fusarium solani; Passion fruit

Controlled infection systems constitute a valuable tool in the study of pathosystems, as they enable observation and measurement specific characteristics with greater precision ease compared to natural conditions. Additionally, are useful for exploring identifying sources resistance host populations. However, plant's response can vary depending on inoculation method; invasive methods damage plant parts, thus facilitating pathogen entry even presence certain mechanisms. The purpose this was evaluate reproducibility an artificial protocol assessment scale primary symptoms at stem base/root collar, well secondary leaves roots two Passiflora species. We evaluated effects inoculating planting substrates sweet granadilla purple passion fruit plants three concentrations F. solani f. sp. passiflorae (0.5 × 106, 1 2 106 conidia gram-1 substrate), using non-inoculated controls under controlled These treatments were tested separate experiments performed 2017 2020. included symptom development readings integrated disease severity index (SI) collar rot. Both showed indices that 56%, indicating high level efficacy system replicating greenhouse settings. Our method reflects conditions plants; it could be suitable epidemiological control studies, routine germplasm screening identify resistance.