MOLECULAR ANALYSIS OF SOME FORENSICALLY SIGNIFICANT DIPTERANS ASSOCIATED WITH DECOMPOSING PIG (SUS SCROFA) CARCASS IN IWO SOUTH-WESTERN NIGERIA USING THE COI GENE

• DOI: 10.22452/mjs.vol43no2.1
• Publication Date: 2024-07-03T03:30:31Z
• Authors: Iyabo Victoria Ol...; Wasiu Adesola Muse; Olusesan Adeyemi ...; Olufemi Michael A...; Anthony Ifeanyi Okoh
• Keywords: Domestic pig

Identification of many forensically important insects has been achieved using morphological keys with only a few relying on molecular techniques. Yet, information regarding the identification Dipteran flies from sub-Saharan Africa is scarce. Therefore, this study used COI gene to analyze fly specimens collected decomposing pig (Sus scrofa) carcasses in Iwo South-Western Nigeria. Several batches eggs were reared adults and preserved 75% ethanol for subsequent techniques triplicate. A total 450 bp sequences was obtained BLAST analysis samples population, which led four families consisting five species following breakdown: Sarcophaga africa (95.4%), Chrysomya putoria (99.1%), inclinata (98.3%), Tricogena rubricosa (89.6%), Chirosia flavipennis (88.8%). The phylogenetic identified chloropyga (AY139694) as same C. S. cruentata, suggesting them sister species. This concluded that T. are implicated carrion decomposition, provides crucial insights forensic investigations