High-throughput quantitative analysis of mitotic defects in fission yeast using Imaging Flow Cytometry

• DOI: 10.22541/au.169749903.33504559/v1
• Publication Date: 2023-10-16T23:30:35Z
• Authors: Martin Prevorovsky; Viacheslav Zemlia...; Jarmila Princová; Martin Báječný
• Keywords: Schizosaccharomyces

In this study, we introduce a novel approach for the high-throughput quantitative analysis of mitotic defects in fission yeast Schizosaccharomyces pombe using Imaging Flow Cytometry (IFC). Fission is valuable model organism cell cycle research, and ‘cut’ phenotype, indicative catastrophe, has been instrumental discovering regulators. Traditional fluorescence microscopy methods quantifying events suffer from subjectivity limited throughput. Our IFC pipeline overcomes these limitations by automating detection cells based on unique characteristic daughter nuclei becoming trapped wall during aberrant mitosis. We demonstrate pipeline’s effectiveness wild-type mutant strains, with results validated against manual scoring. study establishes as powerful tool investigating fidelity yeast, implications advancing biology research.