Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern Asplenium ceterach L.

• DOI: 10.2298/abs1004071z
• Publication Date: 2011-01-17T13:37:09Z
• Authors: Suzana Zivkovic; M. Popovic; Jelena Dragisic-M...; Ivana Momcilovic; D. Grubisic
• Keywords: Frond

Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated state. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate loss (partial desiccation) fern tissue. The main phenolic acids represented A. were chlorogenic (CGA) caffeic acid (CA) their content decreased during the process. For first time, peroxidase (POD) polyphenol oxidase (PPO) isoforms determined rustyback fern. results exhibit presence numerous anionic POD isoforms, with pI ranging 4.4 5.8, but none cationic was detected. Two PPO identified, one 6.3 about 9.0. Short-term brought remarkable increase activity using CGA as substrate. Changes enzyme substrates may play important role adaptation deficit, overall plant resistance stress conditions.