Variations in Microsatellite Sequences Provide Evidence for Population Differences and Multiple Ribosomal Gene Repeats within Trichinella pseudospiralis

• DOI: 10.2307/3283777
• Publication Date: 2006-05-06T14:13:38Z
• Authors: Dante S. Zarlenga; Richard A. Aschen...; J. Ralph Lichtenfels
• Keywords: genomic DNA; Trichinella

Enzymatic amplification of expansion segment 5 sequences within domain IV the large subunit ribosomal DNA generated distinct results among geographical isolates Trichinella pseudospiralis from Russia, North America, and Australia both avian mammalian hosts. Discrete, multiple fragments ranging in approximate size 285 to 360 bp were observed each tested. Polymerase chain reaction performed on individual adult parasites isolate resulted that comparable those pooled genomic DNA. Sequence analysis cloned, representative amplified demonstrated fragment length variation primarily dinucleotide (TG)n trinucleotide (TGC)n microsatellite repeats present segment. Results are consistent with population differences species as well presence alleles RNA genes parasites.