Description of a New Neospora Species (Protozoa: Apicomplexa: Sarcocystidae)

• DOI: 10.2307/3284632
• Publication Date: 2006-05-06T10:33:17Z
• Authors: Antoinette E. Marsh; Bradd C. Barr; Andrea E. Packham; Patricia A. Conrad
• Keywords: Conoid; Rhoptry; Neospora

Neospora hughesi n. sp. was isolated from the central nervous system tissue of an adult equine (Equus caballus) California. The tachyzoites are crescent-shaped, approximately 2 x 5 microm (1.8-3.0 4.0-7.0 microm), with characteristic apical complex structures consisting anterior polar ring, conoid, numerous rhoptries filled a uniform electron-dense material, and 22 microtubules extending posteriorly ring. Comparison N. to canine bovine caninum isolates showed phenotypic differences in immunoreactive proteins. Molecular analysis small subunit ribosomal RNA gene revealed no nucleotide sequence between examined. However, internal transcribed spacer I region 7 base (CN1 BPA1) analyzed this study. existence regions suggests that may be genetic marker for discriminating species within genus Neospora. ultrastructural, antigenic, molecular data support distinction as new species, separate caninum, only recognized genus.