Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences

• DOI: 10.1080/00275514.1995.12026524
• Publication Date: 2018-08-30T14:46:26Z
• Authors: Jean-Marc Moncalvo; Hsi-Hua Wang; Ruey-Shyang Hseu
• Keywords: Subgenus; Monophyly; Spacer DNA

Over 250 species have been described in Ganoderma. Species identification and circumscription are often unclear taxonomic segregation of the genus remains controversial. In this study we sequenced 5′ half 25S ribosomal RNA gene internal transcribed spacers to determine appropriate regions i) discriminate between Ganoderma ii) infer s. lato (Ganodermataceae) on a phylogenetic basis. We studied 19 isolates representing 14 classified 5 subgenera sections, one isolate related Amauroderma, Fomitopsis which served as out- group parsimony analysis. Results showed that transition bias was present our data, rates nucleotide divergence different varied lineages. Independent combined analyses data sets were performed results discussed. Nucleotide sequences spacers, but not those coding regions, distinguished most species, indicated G. tsugae misnamed. Phylogenetic analysis divergent domain D2 subgenus Elfvingia monophyletic, whereas sections Characoderma Phaeonema not. Combined from these is useful for infrageneric region alone strongly supported Amauroderma sister taxon This suggested should be suitable systematics at higher ranks Ganodermataceae. The low sequence variation observed within young.