Differential Expression of PAI-RBP1, C1orf142, and COTL1 in Non–Small Cell Lung Cancer Cell Lines with Different Tumor Metastatic Potential

• DOI: 10.2310/jim.0b013e31824963b6
• Publication Date: 2016-05-11T13:10:57Z
• Authors: Wenjing Sun; Changlong Guo; Xiangning Meng; Yang Yu; Yan Jin; Dandan Tong; Jingshu Geng; Qi Huang; Jiping Qi; An Liu; Rongwei Guan; Lidan Xu; Donglin Sun; Wei Ji; Peng Liu; Fangli Liu; Haiming Sun; Guohua Ji; Songbin Fu; Jing Bai
• Keywords: Gene Expression Regulation, Neoplastic; 0303 health sciences; 03 medical and health sciences; Lung Neoplasms; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Microfilament Proteins; Humans; RNA-Binding Proteins; Qc-SNARE Proteins; Qb-SNARE Proteins; 3. Good health

Human non–small cell lung cancer (NSCLC) is one of the most common malignancies in modern world. Its recurrence mainly due to its ability invade and metastasize. However, precise mechanism for tumor development metastasis still not fully understood. To shed light on cancer, human giant carcinoma lines 95D with high metastatic potential 95C low were selected this study. The 2 originated from same parental share a similar genetic background. In current study, we identified 3 differentially expressed proteins lines, namely, PAI-RBP1, C1orf142, COTL1, by using 2-dimensional electrophoresis proteomics analysis. We found that PAI-RBP1 C1orf142 expression levels higher than cells, whereas COTL1 level was lower when compared cells. also confirmed these results reverse transcription–polymerase chain reaction immunoblotting analyses. messenger RNA protein much assessed immunohistochemistry 70 NSCLC 7 normal tissue samples patients. tissues (positive staining 87.1% cases [61/70]) 14.3% [1/7]). conclusion, studying as well tissues, have proteins, which different potential. addition, might contribute development.