GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity

• DOI: 10.2323/jgam.57.207
• Publication Date: 2011-09-13T07:00:10Z
• Authors: Masato Kawabe; Akiko Okabe Onokubo; Yutaka Arimoto; Takanobu Yoshida; Koji Azegami; Tohru Teraoka; Tsutomu Arie
• Keywords: 0301 basic medicine; Virulence; Chromosome Mapping; Brassica; Plant Roots; Fungal Proteins; Two-Dimensional Difference Gel Electrophoresis; 03 medical and health sciences; Fusarium; Seedlings; Mutation; Cloning, Molecular; DNA, Fungal; Oxidoreductases; Pest Control, Biological; Phylogeny; Plant Diseases

A spontaneous non-pathogenic variant (Cong:1-2) derived from Fusarium oxysporum f. sp. conglutinans (Cong: 1-1), a causal agent of cabbage yellows, carries biocontrol activity for yellows. We found GMC oxidoreductase (ODX1) among the proteins expressed much more in Cong:1-2 than Cong:1-1 by 2D-DIGE comparison. oxidoreductases have been reported to be involved several plant pathogenic fungi. The gene encoding ODX1 was cloned, and targeted disruption did not affect its activity, suggesting that is dispensable fungal agent.