Differentiation of the human mesenchymal stem cells derived from bone marrow and enhancement of cell attachment by fibronectin

• DOI: 10.2334/josnusd.46.207
• Publication Date: 2005-11-29T05:57:18Z
• Authors: Naomi Ogura; Masaru Kawada; Wei-Jen Chang; Qi Zhang; Sheng-Yang Lee; Toshirou Kondoh; Yoshimitsu Abiko
• Keywords: Von Kossa stain; ALIZARIN RED

The ability of human mesenchymal stem cells (hMSC) to differentiate into osteoblasts was examined through the use osteogenic induction medium (MSCOIM) cultures. hMSC first attached dish surface and exhibited fibroblast-like spindle shapes, after proliferation, formed cuboidal shapes. Calcium assays von Kossa alizarin red S staining showed that were capable mineralization when cultured in MSCOIM. Gene expressions Cbfa-1 BMP-4, which are markers for differentiation, also increased during differentiation osteoblasts. When compared albumin (Alb)-coated dishes, microscopic observation documented enhanced cell attachment spreading on fibronectin (FN)-coated dishes. Adherent numbers a greater increase FN-coated dishes earlier culture stages than seen Alb-coated These findings suggest have capability FN can stimulate hMSC.