Differentiation of the human mesenchymal stem cells derived from bone marrow and enhancement of cell attachment by fibronectin
0303 health sciences
Osteoblasts
Cell Culture Techniques
Bone Marrow Cells
Cell Differentiation
Mesenchymal Stem Cells
Serum Albumin, Bovine
Bone Morphogenetic Protein 4
Polymerase Chain Reaction
Fibronectins
Neoplasm Proteins
03 medical and health sciences
Bone Morphogenetic Proteins
Cell Adhesion
Humans
Calcium
Cells, Cultured
Transcription Factors
DOI:
10.2334/josnusd.46.207
Publication Date:
2005-11-29T05:57:18Z
AUTHORS (7)
ABSTRACT
The ability of human mesenchymal stem cells (hMSC) to differentiate into osteoblasts was examined through the use of osteogenic induction medium (MSCOIM) cultures. hMSC first attached to the dish surface and exhibited fibroblast-like spindle shapes, and after proliferation, formed cuboidal shapes. Calcium assays and the use of von Kossa and alizarin red S staining showed that hMSC were capable of mineralization when cultured in MSCOIM. Gene expressions of Cbfa-1 and BMP-4, which are markers for osteogenic differentiation, were also increased during the hMSC differentiation into osteoblasts. When compared to albumin (Alb)-coated dishes, microscopic observation documented enhanced cell attachment and spreading when hMSC were cultured on fibronectin (FN)-coated dishes. Adherent cell numbers also exhibited a greater increase on the FN-coated dishes during earlier culture stages than that seen for the Alb-coated dishes. These findings suggest that hMSC have the capability to differentiate into osteoblasts and that FN can stimulate the attachment and spreading of the hMSC.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (23)
CITATIONS (86)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....