Obesity Blunts Insulin-Mediated Microvascular Recruitment in Human Forearm Muscle

• DOI: 10.2337/db05-1373
• Publication Date: 2006-05-10T22:48:46Z
• Authors: Lucy H. Clerk; Michelle A. Vincent; Linda A. Jahn; Zhenqi Liu; Jonathan R. Lindner; Eugene J. Barrett
• Keywords: Brachial artery; Glucose clamp technique

We have previously shown that skeletal muscle capillaries are rapidly recruited by physiological doses of insulin in both humans and animals. This facilitates glucose delivery to muscle, thus augmenting uptake. In obese rats, insulin-mediated microvascular recruitment uptake diminished; however, this action has not been studied humans. Here we used contrast ultrasound measure blood volume (MBV) (an index recruitment) the forearm flexor muscles lean adults before after a 120-min euglycemic-hyperinsulinemic (1 mU · min−1 kg−1) clamp. also measured brachial artery flow, fasting lipid profile, anthropomorphic variables. Fasting plasma (5.4 ± 0.1 vs. 5.1 mmol/l, P = 0.05), (79 11 38 6 pmol/l, 0.003), percent body fat (44 2 25 2%, 0.001) were higher than adults. After h infusion, whole-body infusion rate was significantly lower versus group (19.3 3.2 37.4 2.6 μmol kg−1 respectively, < 0.001). Compared with baseline, increased MBV (18.7 3.3 25.0 4.1, 0.019) but (20.4 3.6 18.8 3.8, NS). Insulin diameter flow group. observed significant, negative correlation between ΔMBV BMI (R −0.482, 0.027) response insulin. conclusion, obesity eliminated insulin-stimulated seen individuals.