Cardioprotective and Anti-Inflammatory Effects of Interleukin Converting Enzyme Inhibition in Experimental Diabetic Cardiomyopathy

• DOI: 10.2337/db06-1662
• Publication Date: 2007-06-27T16:33:45Z
• Authors: Dirk Westermann; Sophie Van Linthout; Sameer Dhayat; Nasser Dhayat; Felicitas Escher; Carola Bücker-Ga...; Frank Spillmann; Michel Noutsias; Alexander Riad; Heinz-Peter Schul...; Carsten Tschöpe
• Keywords: Diabetic Cardiomyopathy

We investigated the effect of pharmacological inhibition interleukin converting enzyme (ICE) on cardiac inflammation, apoptosis, fibrosis, and left ventricular function in an animal model diabetes.Diabetes was induced 24 Sprague-Dawley rats by injection streptozotozin (STZ) (70 mg/kg). Diabetic animals were treated with inhibitor (ICEI) (n = 12) or a placebo 12). Nondiabetic served as controls Left documented 6 weeks after induction diabetes. Cardiac tissue analyzed for expression cytokines, intracellular adhesion molecule-1 vascular cell molecule-1, leukocyte macrophage integrins, collagen. Phosphorylation Akt Western blot apoptosis Blc-2 Bax measurements.Left significantly impaired diabetic animals. This accompanied significant increase molecules, leukocytes macrophages, collagen content. In addition, phosphorylation state reduced. These changes attenuated group ICEI.Cardiac dysfunction is associated inflammation experimental cardiomyopathy. Both these--cardiac inflammation--are treatment ICEI. data suggest that anticytokine-based therapies might be beneficial