Development of the Human Pancreas From Foregut to Endocrine Commitment

• DOI: 10.2337/db12-1479
• Publication Date: 2013-04-30T03:22:22Z
• Authors: Rachel E. Jennings; Andrew A. Berry; Rebecca Kirkwood-...; Neil A. Roberts; Thomas Hearn; Rachel J. Salisbury; Jennifer Blaylock; Karen Piper Hanley; Neil A. Hanley
• Keywords: Foregut; Enteroendocrine cell; PAX6; PDX1; Cell fate determination; Fate mapping; Developmental Biology; Directed differentiation

Knowledge of human pancreas development underpins our interpretation and exploitation pluripotent stem cell (PSC) differentiation toward a β-cell fate. However, almost no information exists on the early events pancreatic specification in distal foregut, bud formation, development. Here, we have studied expression profiles key lineage-specific markers to understand morphogenetic during The notochord was adjacent dorsal foregut endoderm fourth week before duodenal homeobox-1 detection. In contrast published data from mouse embryos, development, detected only single-phase Neurogenin 3 (NEUROG3) endocrine approximately 8 weeks, which Nirenberg Kim homeobox 2.2 (NKX2.2) not observed progenitor population. addition revealing number disparities timing between these data, directly assembled tissue, allow combinations transcription factors define sequential stages differentiating types. are anticipated provide useful reference point for researchers looking differentiate PSCs vitro so as model or enable drug discovery potential therapy.