RNA-Binding Protein PTB and MicroRNA-221 Coregulate AdipoR1 Translation and Adiponectin Signaling

• DOI: 10.2337/db13-1032
• Publication Date: 2013-10-16T04:44:29Z
• Authors: Yaniv Lustig; Ehud Barhod; Reut Ashwal-Fluss; Reut Gordin; Noam Shomron; Kfir Baruch-Umansky; Rina Hemi; Avraham Karasik; Hannah Kanety
• Keywords: Adiponectin receptor 1

Adiponectin receptor 1 (AdipoR1) mediates adiponectin's pleiotropic effects in muscle and liver plays an important role the regulation of insulin resistance diabetes. Here, we demonstrate a pivotal for microRNA-221 (miR-221) RNA-binding protein polypyrimidine tract-binding (PTB) posttranscriptional AdipoR1 during differentiation obesity. RNA-immunoprecipitation luciferase reporter assays illustrated that both PTB miR-221 bind AdipoR1-3'UTR cooperatively inhibit translation. Depletion or increased, while overexpression these factors decreased, synthesis cells. During myogenesis, downregulation robustly induced translation, providing mechanism enhanced expression activation differentiated In addition, since are upregulated genetic dietary mouse models obesity, this novel translational may be at least partly responsible reduction levels These findings highlight importance control regulating adiponectin signaling. Given is reduced induction could potentially enhance beneficial ameliorate