Mannose-Binding Lectin Is Required for the Effective Clearance of Apoptotic Cells by Adipose Tissue Macrophages During Obesity

• DOI: 10.2337/db14-0256
• Publication Date: 2014-07-10T07:04:07Z
• Authors: Rinke Stienstra; Wieneke Dijk; Lianne van Beek; Henry Jansen; Mattijs Heemskerk; Riekelt H. Houtko...; Simone Denis; Vanessa van Harmelen; Ko Willems van Dijk; Cees J. Tack; Sander Kersten
• Keywords: Adipose tissue macrophages; Stromal vascular fraction; Macrophage-activating factor

Obesity is accompanied by the presence of chronic low-grade inflammation manifested infiltration macrophages into adipose tissue. Mannose-binding lectin (MBL), a soluble mediator innate immunity, promotes phagocytosis and alters macrophage function. To assess function MBL in development obesity, we studied wild-type MBL(-/-) mice rendered obese using high-fat diet (HFD). Whereas no gross morphological differences were observed liver, an HFD provoked distinct changes tissue morphology mice. In parallel with increased adipocyte size, displayed influx Macrophages polarized toward alternatively activated phenotype known to modulate apoptotic cell clearance. deficiency also significantly number cells Consistent these observations, recombinant enhanced phagocytic capacity stromal vascular fraction isolated from modulated uptake adipocytes macrophages. Despite abundance polarity, absence did not affect systemic insulin resistance. Finally, humans, lower levels circulating subcutaneous We propose novel role for recognition clearance during obesity.