Improved Insulin Sensitivity 3 Months After RYGB Surgery Is Associated With Increased Subcutaneous Adipose Tissue AMPK Activity and Decreased Oxidative Stress

• DOI: 10.2337/db14-1765
• Publication Date: 2015-05-23T02:41:48Z
• Authors: X. Julia Xu; Caroline Apovian; Donald Hess; Brian Carmine; Asish Saha; Neil Ruderman
• Keywords: Gastric bypass surgery

Morbidly obese individuals are predisposed to a wide range of disorders, including type 2 diabetes, atherosclerotic cardiovascular disease, fatty liver and certain cancers. Remarkably, all these disorders can be improved or prevented by Roux-en-Y gastric bypass (RYGB) surgery. We have reported that decreased AMPK activity, together with increased oxidative stress inflammation in adipose tissue, is associated insulin resistance morbidly bariatric surgery patients. In the current study, we assessed how parameters affected RYGB Eleven patients (average age 46 ± 4 years) were studied immediately prior 3 months postoperatively. measured subcutaneous tissue phosphorylation (threonine 172, an index its activation), malonyl-CoA content, protein carbonylation (a marker stress), plasma adiponectin, mRNA expression several inflammatory cytokines. After surgery, activity 3.5-fold 50% tissue. addition, levels reduced 80%. Furthermore, had improvements their BMI sensitivity (HOMA) circulating high–molecular weight adiponectin fasting levels. contrast, markers was unchanged postoperatively, although CRP diminished 50%.