Increased Interleukin-32 Levels in Obesity Promote Adipose Tissue Inflammation and Extracellular Matrix Remodeling: Effect of Weight Loss

• DOI: 10.2337/db16-0287
• Publication Date: 2016-09-15T02:18:35Z
• Authors: Victoria Catalán; Javier Gómez-Ambrosi; Amaia Rodríguez; Beatriz Ramírez; Víctor Valentí; Rafael Moncada; Manuel F. Landecho; Camilo Silva; Javier Salvador; Gema Frühbeck
• Keywords: Monocyte; Adipose tissue macrophages

Interleukin (IL)-32 is a recently described cytokine involved in the regulation of inflammation. We aimed to explore whether IL-32 could function as an inflammatory and angiogenic factor human obesity obesity-associated type 2 diabetes. Samples obtained from 90 subjects were used study. Obese patients exhibited higher expression levels visceral adipose tissue (AT) well subcutaneous AT peripheral blood mononuclear cells. IL32 was mainly expressed by stromovascular fraction cells, its significantly enhanced stimuli hypoxia, whereas no changes found after incubation with anti-inflammatory cytokines. The addition exogenous induced inflammation extracellular matrix–related genes adipocyte cultures, IL32-silenced adipocytes showed downregulation genes. Furthermore, adipocyte-conditioned media obese increased gene monocyte lean volunteers had effect on mRNA levels. These findings provide evidence, for first time, about remodeling properties AT, implicating this comorbidities.